BIOCHEMISTRY LABORATORY I
BCHS 3201
Fall 2013
Lab Coordinator: Dr.
Donna Pattison
Office: Room 108C,
Science Teaching Laboratories Building
Phone Number:
713-743-2642
e-mail: dpattison@uh.edu
Office hours: Monday
and Wednesday 9-10 am or by appointment
Teaching Assistant:________________________________
TA e-mail:_______________________________________
Text: Purchased from Research Stores on the second
floor of the Science Building. A
biochemistry textbook will be very helpful to you as you write your lab
reports. The Voet, Voet, Pratt used for
BCHS3304 is recommended.
A calculator may be
helpful. Lab coats and goggles are
REQUIRED. You will also need a tear-out
lab notebook with duplicate pages. Goggles, lab coats and notebooks are
available for purchase at Research Stores on the second floor of the Science
Building.
Course Description: This course reviews the nature of the
chemical constituents of living organisms, including nucleic acids and protein. Students will gain hands-on experience in
common lab techniques used to study nucleic acids, proteins and enzyme
activity.
Objectives:
Students will:
1) demonstrate basic skills in laboratory math.
2) demonstrate the ability to use common laboratory
equipment such as centrifuges,
pipettors,
chromatography columns, and electrophoresis equipment.
3) be able to
critically analyze and explain their experimental results.
4) apply the
scientific method and their knowledge of biochemistry to design and carry
out experiments.
Week Lab
August 26-29 Lab Orientation and
Safety; Pipet Techniques
September 2-5 Making Solutions and
Determining Your Sugar Tasting
Threshold;
Graphing in Excel (no class Monday,
September
2)
September 9-12 Plasmid Isolation
Quiz 1
September 16-19 Restriction Digests of DNA
and Gel Electrophoresis
Using the Computer in Biochemical Research I: BLAST Search and Sequence Alignment (Homework
due)
September 23-26 Identification of Serum
Glycoprotein by Polyacrylamide Gel Electrophoresis and Western Blotting
Using the Computer in Biochemical Research II: Restriction Digests (Homework due)
September 30-October
3 Fold-It: Computer Assisted Protein Folding Predictions;
Quiz 2
October 7-10 Protein
Crystallization; Crystallography Pre-Lab
Homework Assignment Due; Quiz 3
October 14-17 Size Exclusion
Chromatography; Literature Search
Homework due
October 21-24 Complete Protein
Crystallization lab; Writing Workshop
October 28-31 Chloroplast Extraction
and the Hill Reaction: The
Biochemistry
of Photosynthesis
November 4-7 Crystallography Paper Rough Draft due;
Peer review
editing
session; Quiz 4
November 11-14 Enzyme Kinetics: Turnip Peroxidase
Protein
Crystallization Manuscript due
November 18-21 Enzyme
Kinetics: Turnip Peroxidase (continued)
November 25-28 No labs.
Thanksgiving Holiday
December 2-5 Last lab report due; Quiz 5
*****Please note that the
above schedule is tentative and is subject to change based on the availability
of materials from our suppliers. Your
teaching assistants will inform you of any changes.
Class Policies
Laboratory Attire: Lab
coats are required. You will not be
allowed to attend the lab without a lab coat.
Safety goggles will be required for certain labs. Gloves will be provided as needed. Please inform your instructor if you are
allergic to latex.
Guesting: If you miss a
lab for an excused reason, inform your
TA as soon as possible.
Make arrangements with another TA (see schedule posted on classroom door
and on Blackboard) to guest in their lab.
You may guest up to two times in a semester. Please contact your regular TA and the TA
with whom you will be guesting for permission.
You do NOT need to contact Dr. Pattison for permission. No more than 5 students will be permitted to
guest in a single section. You are also
required to fill out the guesting form (located on the TA bench at the front of
the room) when you arrive for the section in which you are guesting. There
are no labs on Friday. You will take the quiz for the lab section you are
visiting.
Absences: Attendance is MANDATORY.
As the main point of a laboratory course is to gain hands-on experience in lab
techniques, if you fail to attend more than two labs, you will automatically
fail the course unless you provide evidence of extenuating circumstances that
are excused under university policies. It
is YOUR responsibility to
deal with attendance issues PROMPTLY. If there are extenuating circumstances and
you have already guested twice, you must speak with both your TA AND Dr.
Pattison.
Late work: Failure to hand in 2 or more lab reports will result
in an F even if you attended the lab. Lab
reports are due at the start of class.
Reports will be subjected to a ten percent loss in points for failure to
turn the report in at the start of class and for each day beyond the due date.
Lab reports that are more than 7 days late will receive a zero.
Arriving Late: You are expected to arrive in class on
time. You will not receive additional
time to complete a quiz if you are late.
Points will be deducted from your participation grade for tardiness. If you
are more than 15 minutes late, you are considered absent and will not be
allowed to participate in the lab.
You will need to guest in another lab to obtain credit for that
lab.
Incomplete Grade Policy: A temporary grade of “I” can be assigned by
the instructor when a student is currently (A) passing a course or (b) still
has a reasonable chance of passing in the judgment of the instructor, but for
non-academic reasons beyond their control have not completed a relatively small
part of all course requirements. After the student and the instructor agree
that the student shall receive an “I” grade, an “Incomplete Grade Agreement”
form must be completed and filed with the Office of Undergraduate Affairs
(124F, Science Building
Students with Special Needs:
The Americans with Disabilities Act of 1990 requires that
universities make reasonable accommodations to persons with disabilities as
defined in the act. Students who feel
they need assistance as defined by the guidelines set forth in the act should
contact the lab coordinator, Dr. Pattison, to discuss appropriate arrangements.
Important Dates:
September 11: Last
day to drop class without a grade
November 1: Last day
to drop class with a “W”
Grades
Lab Activities and
Reports (45%)
Pipet technique (No lab report due)
Making Solutions/Sugar Sensing
DNA Plasmid Purification
DNA Restriction Digests
Polyacrylamide Gels
Fold-It
Size Exclusion Chromatography
Hill Reaction
Enzyme Kinetics
Journal Style Lab
Manuscript (15%)
Crystallization
Quizzes (5)
(30%)
Homework
(4) (7%)
Literature Search
Using the Computer in Biochemical Research I: BLAST Search/Sequence Alignment
Using the Computer in Biochemical Research II: Restriction Digests
Crystallography Pre-Lab Homework
Participation (3%)
Grade Assignments
% Letter
Grade
92.0 – 100% A
90.0 - 91.9% A-
88.0 - 89.9% B+
82.0 – 87.9% B
80.0 – 81.9% B-
78.0 – 79.9% C+
72.0 – 77.9% C
70.0 – 71.9% C-
68.0 – 69.9% D+
62.0 – 67.9% D
60.0 – 61.9% D-
≤59.9% F
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